behnaz bageshlooyafshar; Reza Rahchamani; Abdollah Mohammadi-Sangcheshmeh; Ehsan Seyedjafari; Yussof Mostafaloo
Volume 20, Issue 2 , August 2018, , Pages 339-349
Abstract
This study was conducted to investigate the differentiation potential of equine adipose-derived mesenchymal stem cell into bone in single-dimensional culture system (in plastic tissue culture) and in three-dimensional system (on poly-l-lactic acid scaffolds; PLLA). A porous structure that allows use ...
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This study was conducted to investigate the differentiation potential of equine adipose-derived mesenchymal stem cell into bone in single-dimensional culture system (in plastic tissue culture) and in three-dimensional system (on poly-l-lactic acid scaffolds; PLLA). A porous structure that allows use of three-dimensional distribution and provides optimal growth of cells is of great clinical significance in the field of tissue engineering. In current study using equine adipose-derived stem cells (ASCs), we intended to compare the osteogenic differentiation potential of PLLA nanofibrous scaffold with tissue culture plastic (TCP). Adipose tissues were collected from 3 adult horses, and ASCswere isolated by enzymatic digestion. PLLA nanofibrous scaffold was successfully prepared using a phase separation method. Viability and growth characteristics of ASCs on TCP and scaffold were investigated by tetrazolium (MTT) based colorimetric assay. Alizarin Red staining was performed for determination of calcium deposition following osteogenic differentiation. Furthermore, other common osteogenic markers such as alkaline phosphatase (ALP) activity, and calcium content were also analyzed. Our data showed that the PLLA scaffold had no detrimental effect on the cell growth rate as evaluated by MTT assay. However, ASCs that differentiated on PLLA nanofibrous scaffolds indicated higher ALP activity and more calcium content than that on TCP. Adequate proliferation rate and higher expression of osteogenic markers of stem cells cultured on PLLA nanofibrous scaffolds provide this scaffold as a suitable substrate to support proliferation and differentiation of ASCs in equine.
Mohammad Heidarian; JAvad Bayad Koohsar; Youssef Moostafloo; Balal Sadeghi; Farid Moslemipoor
Volume 18, Issue 3 , October 2016, , Pages 461-475
Abstract
In order to compare the effects of different weaning strategies on feed intake, structural growth measurement and health, blood metabolites and economic efficiency of young dairy calves, twenty four female Holstein calves were used. Calves after birth were randomly assigned into one of three weaning ...
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In order to compare the effects of different weaning strategies on feed intake, structural growth measurement and health, blood metabolites and economic efficiency of young dairy calves, twenty four female Holstein calves were used. Calves after birth were randomly assigned into one of three weaning strategy as follow: 1) weaning based on feed intake, 2) weaning based on weight gain (30 kg weight gain compared to the birth weight), and 3) weaning based on age (at 90 days of age). Starter intake measurement and fecal scoring conducted daily. Calves were weighed weekly and blood samples were obtained on days 21, 42, 60 and 90 after birth. Results showed that there were significant differences among different weaning strategies on average weaning age and milk consumption (p<0.05). All treatments had similar average daily weight gain, average daily feed intake, final weight and structural growth measurements. Calves weaned based on feed intake had lowest ruminal pH and N-NH3 concentration and blood urea nitrogen on day 90 (p<0.05). There were differences between groups on milk cost and total cost (milk + feed) and feed cost per kg of BW gain, so that Weaning based of feed intake compared to weaning based on weight and age saved up to 6000 and 3000 Rials per kg of body weight, respectively. Generally, similar growth and performance among different weaning strategies as well as reduction the cost of raising of calves indicated that calves weaning based on feed intake can be profitable approach.